Retry the protocol with freshly manufactured dialysis buffer, staying incredibly mindful to add the right amount of NaCl The subsequent write-up describes different disruption methods, and indicates which method to implement for certain tissues/cell types. All of the disruption procedures described Here i will discuss appropriate with Ambion's RNA isolation https://www.facebook.com/NewTechFind/posts/pfbid02RiZtF2CYQRNXKgPH6trquBJHCScc84k1QCRwYeyZWmLUNxJHv6cRNfFN7v8cEgq9l